CSS And Javascripts Are Not Loading When I Host Application In IIS7

I am trying to host asp.net application in IIS7. I am using published version of application. when i run it all css and javascripts are not loading. the strange is when i run from

Solution 1:

The problem is with the IIS,It is not serving Static Content, which you can set up as diplayed in the image

if you have checked that and it still does not work then what you need to do is set the identity of the anonymous authentication to the Application Pool Identity if it is on specific user here is an image of where that is.